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Alginate immobilized algal beads as biomonitors
of metals and nutrients in Hong Kong streams
C.G.Simpson*, N.F.Y. Tam,
*Department of Biology and Chemistry, City University
of Hong Kong; 83 Tat Chee Avenue, Kowloon, Hong Kong SAR,
China; Telephone 852 2784-4660, Fax 852 2788-7406
Email Craig.Simpson@plink.cityu.edu.hk
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Immobilized algal beads were utilized
to monitor nutrients and metals in several streams around Hong Kong.
Cells of Chlorella vulgaris, a single celled microalga approximately
3 - 6 µm in diameter, were immobilized in alginate beads with
a density of approximately 1 x 106
cells bead-1. The algal beads
were deployed for five days in a variety of Hong Kong streams ranging
from pristine hillside streams to a rural stream heavily polluted
with piggery wastewater. Algal growth was monitored through changes
in chlorophyll a and cell number. The changes in chlorophyll a bead-1
was found to be a more suitable parameter to indicate nutrient status
of streams than cells bead-1.
Increases in chlorophyll a bead-1
ranged from 72 % in pristine streams (ortho P concentration <
0.01 mg 1-1) to 876 % in a nutrient
enriched stream (mean ortho P concentrations of 0.48 mg 1-1).
Significant positive correlations were found between the log values
of percentages increase in chlorophyll a bead-1
and log ortho P concentrations in stream water (r = 0.811, P <
0.01). Moreover, log values of percentages increase in chlorophyll
a bead-1 were inversely proportional
to log inorganic N:ortho P atomic ratios of stream water (r = -
0.814, P < 0.05). Increases in algal cells bead-1
ranged from no change to 478 % but were not correlated with any
nutrient concentration. In terms of metal monitoring, algal beads
adsorbed metals during stream exposures and Al, Cu, Ni and Zn were
all adsorbed and concentrated by algal beads. The metals adsorbed
onto the algal beads were desorbed and recovered by placing the
exposed beads in 0.1 M HCl solution, where the low pH caused the
bound metals to be released from the beads into the HCl solution.
The recovered metals were more concentrated in the HCl compared
to their concentrations in stream water, which allowed easy analysis.
The concentration factors of the algal beads, in all the streams,
were Al 1268 - 31327, Cu 924 - 25224, Ni 733 - 808, and Zn 9866
- 29570, and these values were comparable to those reported in the
literature employing similar biomonitors, such as macroalgae. High
concentrations of Cu and Ni were adsorbed by algal beads exposed
in one stream, Tai Wai Nullah, with the values of 926 µg Cu
g-1 dry wt. algal bead and 120
µg Ni g-1 dry wt. algal
bead. In this particular stream the concentrations of dissolved
Cu and Ni in stream water were 102 µg 1-1
and 149 µg 1-1, respectively.
The metal adsorbed did not seem to have any toxic effects on the
immobilized algal cells, nor did immobilized cells affect metal
adsorption by beads. However the alginate matrix deteriorated to
varied extents in streams, ranging from minor changes to complete
dissolution of the alginate beads. Ortho P appeared responsible
for the degradation of alginate matrix and shrinkage of beads started
to appear when ortho P concentration exceeded 0.5 mg 1-1.
A significant negative correlation was found between bead deterioration
(indicated by reduction in dry weight of algal beads after five
days stream exposures) and ortho P concentrations (r = - 0.711,
P < 0.05). This study demonstrates that alginate immobilized
algal beads could be used to indicate nutrient status of streams
in Hong Kong through monitoring changes in chlorophyll a
bead-1 over five days exposure.
At the same time, metals adsorbed onto the alginate beads provided
a good indication on degree of metal contamination in stream water.
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